Hotspot

rpoA

LSC · 79946-81010 · 1065 bp

Back to Saurauia

Pi 0.00376

MarkerSeek score 60.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1065
variable_sites: 4
pi: 0.00376
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AAAATCCGCATTGCATTGGA	CCCCATAATGGCTGTAGACC	40.0	55.0	56.9	57.4	1198 bp	0.5	47.0
rpoA_p2	AGAAAATCCGCATTGCATTGG	CCCCATAATGGCTGTAGACC	42.9	55.0	58.1	57.4	1200 bp	0.5	46.6
rpoA_p3	GAAAATCCGCATTGCATTGGA	CCCCATAATGGCTGTAGACC	42.9	55.0	58.1	57.4	1199 bp	0.5	46.6
rpoA_p4	AAATCCGCATTGCATTGGAT	CCCCATAATGGCTGTAGACC	40.0	55.0	56.7	57.4	1197 bp	0.5	45.2
rpoA_p5	GAAAATCCGCATTGCATTGG	CCCCATAATGGCTGTAGACC	45.0	55.0	56.6	57.4	1199 bp	0.5	44.4