Hotspot

rbcL-accD

LSC · 58405-60167 · 1763 bp

Back to Saurauia

Pi 0.00342

MarkerSeek score 53.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 1763
variable_sites: 6
pi: 0.00342
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCTGCTTGTGAGGTATGGAA	AATCTGCTCGCAACGATTCT	50.0	45.0	57.9	58.0	1868 bp	1.0	79.5
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	AGAAATCTGCTCGCAACGAT	50.0	45.0	57.9	58.0	1871 bp	1.0	79.5
rbcL-accD_p3	GCTGCTTGTGAGGTATGGAA	ATCTGCTCGCAACGATTCTT	50.0	45.0	57.9	58.0	1867 bp	1.0	79.5
rbcL-accD_p4	GCTGCTTGTGAGGTATGGAA	TGAGAAGGCTCCTCCTCAAA	50.0	50.0	57.9	58.0	1936 bp	1.0	79.4
rbcL-accD_p5	GCTGCTTGTGAGGTATGGAA	TTGAGAAGGCTCCTCCTCAA	50.0	50.0	57.9	58.0	1937 bp	1.0	79.4