Hotspot

ndhC-trnV (UAC)

LSC · 51939-53162 · 1224 bp

Back to Saurauia

Pi 0.00664

MarkerSeek score 64.10

Primer available yes

Label ndhC-trnV (UAC)

Feature Metadata

label_name: ndhC-trnV (UAC)
feature_type: igs
region: LSC
length_bp: 1224
variable_sites: 8
pi: 0.00664
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV (UAC)_p1	GGGGCTAAAACTCCGGAAAT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1339-1358 bp	1.0	79.6
ndhC-trnV (UAC)_p2	CTGGTCCTTTGCTAATCGGG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.3	58.1	1356-1375 bp	1.0	78.1
ndhC-trnV (UAC)_p3	CCTTTGCTAATCGGGGCTAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.7	58.1	1351-1370 bp	1.0	78.0
ndhC-trnV (UAC)_p4	TCCTTTGCTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.5	58.1	1352-1371 bp	1.0	76.7
ndhC-trnV (UAC)_p5	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1344-1363 bp	1.0	75.9