Hotspot

trnY (GUA)-trnE (UUC)

LSC · 31363-31426 · 64 bp

Back to Saurauia

Pi 0.0156

MarkerSeek score 61.50

Primer available yes

Label trnY (GUA)-trnE (UUC)

Feature Metadata

label_name: trnY (GUA)-trnE (UUC)
feature_type: igs
region: LSC
length_bp: 64
variable_sites: 1
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY (GUA)-trnE (UUC)_p1	CCAGCGTAGACATATTGCCA	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.8	57.9	207 bp	1.0	77.7
trnY (GUA)-trnE (UUC)_p2	ACCAGCGTAGACATATTGCC	AAGTATGCCCCCATCGTCTA	50.0	50.0	57.8	57.9	208 bp	1.0	77.7
trnY (GUA)-trnE (UUC)_p3	CCAGCGTAGACATATTGCCA	TCTCTTTCAAGGAGGCAACG	50.0	50.0	57.8	57.8	172 bp	1.0	77.2
trnY (GUA)-trnE (UUC)_p4	ACCAGCGTAGACATATTGCC	TCTCTTTCAAGGAGGCAACG	50.0	50.0	57.8	57.8	173 bp	1.0	77.2
trnY (GUA)-trnE (UUC)_p5	CGAATTTACAGTCCGTCCCC	AAGTATGCCCCCATCGTCTA	55.0	50.0	58.4	57.9	186 bp	1.0	76.8