Hotspot

petN-psbM

LSC · 29157-30350 · 1194 bp

Back to Saurauia

Pi 0.00419

MarkerSeek score 61.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1194
variable_sites: 5
pi: 0.00419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTCT	45.0	42.9	58.0	57.6	1349-1358 bp	1.0	69.0
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.8	57.6	1355-1364 bp	1.0	67.5
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1295-1304 bp	1.0	66.2
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTGTTCATTC	45.0	45.0	58.0	56.1	1349-1358 bp	1.0	64.8
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTGTTCATTC	50.0	45.0	57.8	56.1	1355-1364 bp	1.0	63.3