Hotspot

atpA-atpF

LSC · 11805-11860 · 56 bp

Back to Saurauia

Pi 0.0179

MarkerSeek score 62.30

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 1
pi: 0.0179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ACTTGAAGTACGGTACCGGT	TGGTATGTTTGAGGCGATGA	50.0	45.0	58.4	56.9	197-198 bp	1.0	68.8
atpA-atpF_p2	TTGCTCAATACGTTCACGGA	TGGTATGTTTGAGGCGATGA	45.0	45.0	57.6	56.9	150-151 bp	1.0	68.4
atpA-atpF_p3	ACTTGAAGTACGGTACCGGT	TTACGTACCATCAGTGCCAA	50.0	45.0	58.4	56.9	220-221 bp	1.0	68.3
atpA-atpF_p4	ACTTGAAGTACGGTACCGGT	TTTACGTACCATCAGTGCCA	50.0	45.0	58.4	56.9	221-222 bp	1.0	68.3
atpA-atpF_p5	TCGCCTACTTGAAGTACGGT	TGGTATGTTTGAGGCGATGA	50.0	45.0	58.5	56.9	203-204 bp	1.0	68.1