Hotspot

trnG (UCC)-trnR (UCU)

LSC · 9780-10106 · 327 bp

Back to Saurauia

Pi 0.0312

MarkerSeek score 73.00

Primer available yes

Label trnG (UCC)-trnR (UCU)

Feature Metadata

label_name: trnG (UCC)-trnR (UCU)
feature_type: igs
region: LSC
length_bp: 327
variable_sites: 10
pi: 0.0312
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG (UCC)-trnR (UCU)_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	407 bp	1.0	66.7
trnG (UCC)-trnR (UCU)_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	410 bp	1.0	66.4
trnG (UCC)-trnR (UCU)_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	413 bp	1.0	66.0
trnG (UCC)-trnR (UCU)_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	416 bp	1.0	65.7
trnG (UCC)-trnR (UCU)_p5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	57.7	56.9	411 bp	1.0	64.2