Hotspot

trnK (UUU)-rps16

LSC · 3619-4398 · 780 bp

Back to Saurauia

Pi 0.00899
MarkerSeek score 50.90
Primer available yes
Label trnK (UUU)-rps16

Feature Metadata

label_name
trnK (UUU)-rps16
feature_type
igs
region
LSC
length_bp
780
variable_sites
7
pi
0.00899
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK (UUU)-rps16_p1 TACCGTTGAGTTAGCAACCC GGAATTTCGCCCTAATCAAACG 50.0 45.5 57.5 58.3 833-834 bp 1.0 58.6
trnK (UUU)-rps16_p2 TACCGTTGAGTTAGCAACCC TTCGCCCTAATCAAACGAAA 50.0 40.0 57.5 55.7 828-829 bp 1.0 58.5
trnK (UUU)-rps16_p3 TACCGTTGAGTTAGCAACCC TTTCGCCCTAATCAAACGAA 50.0 40.0 57.5 55.7 829-830 bp 1.0 58.5
trnK (UUU)-rps16_p4 TACCGTTGAGTTAGCAACCC TTTCGCCCTAATCAAACGAAA 50.0 38.1 57.5 56.5 829-830 bp 1.0 56.8
trnK (UUU)-rps16_p5 TACCGTTGAGTTAGCAACCC TCGCCCTAATCAAACGAAAT 50.0 40.0 57.5 55.4 827-828 bp 1.0 56.7