Hotspot

rpl2-rps19

IRa · 151677-151736 · 60 bp

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Pi 0.0333

MarkerSeek score 71.70

Primer available yes

Label rpl2-rps19

Feature Metadata

label_name: rpl2-rps19
feature_type: igs
region: IRa
length_bp: 60
variable_sites: 2
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rps19_p1	GATTGTTCGTCGCCGTAGTA	TTTGGCTACAAAAGGATTT	50.0	31.6	57.8	50.2	124 bp	1.0	41.3
rpl2-rps19_p2	TTTGATTGTTCGTCGCCGTA	TTTGGCTACAAAAGGATTT	45.0	31.6	58.2	50.2	127 bp	1.0	41.3
rpl2-rps19_p3	AATTTGATTGTTCGTCGCCG	TTTGGCTACAAAAGGATTT	45.0	31.6	57.8	50.2	129 bp	1.0	41.2
rpl2-rps19_p4	TTGGGGTTATCCTGCACTTG	TTTGGCTACAAAAGGATTT	50.0	31.6	57.8	50.2	184 bp	1.0	40.9
rpl2-rps19_p5	CACAACCCCTTGGGGTTATC	TTTGGCTACAAAAGGATTT	55.0	31.6	58.2	50.2	193 bp	1.0	40.8