Hotspot

rps15-ycf1

SSC · 121293-121711 · 419 bp

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Pi 0.017

MarkerSeek score 51.70

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 419
variable_sites: 7
pi: 0.017
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGTCGTTGACGTTTTCCCAA	TGGGACGCAACTTTGAATCA	45.0	45.0	58.0	58.0	1314-1321 bp	1.0	80.7
rps15-ycf1_p2	CTCGAGTCAGTCTTCCATGC	TGGGACGCAACTTTGAATCA	55.0	45.0	58.1	58.0	1490-1497 bp	1.0	80.2
rps15-ycf1_p3	CTTCACCGTCGAGAGTAACG	TGGGACGCAACTTTGAATCA	55.0	45.0	58.1	58.0	1604-1611 bp	1.0	79.9
rps15-ycf1_p4	CGATACGACTCAACTCCAGC	TGGGACGCAACTTTGAATCA	55.0	45.0	58.2	58.0	1832-1839 bp	1.0	79.3
rps15-ycf1_p5	GCGATACGACTCAACTCCAG	TGGGACGCAACTTTGAATCA	55.0	45.0	58.2	58.0	1833-1840 bp	1.0	79.3