Hotspot

ccsA-ndhD

SSC · 113276-113513 · 238 bp

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Pi 0.0256

MarkerSeek score 64.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 238
variable_sites: 6
pi: 0.0256
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACAAATTCAGCAATTGTAGCCT	TCGGTATTGGTATGTACCCAGA	36.4	45.5	56.9	58.1	444-448 bp	1.0	41.8
ccsA-ndhD_p2	ACAAATTCAGCAATTGTAGCCT	CTCGGTATTGGTATGTACCCAG	36.4	50.0	56.9	57.7	445-449 bp	1.0	41.8
ccsA-ndhD_p3	ACAAATTCAGCAATTGTAGCCT	ACTCGGTATTGGTATGTACCCA	36.4	45.5	56.9	58.4	446-450 bp	1.0	41.8
ccsA-ndhD_p4	ACAAATTCAGCAATTGTAGCCT	CGGTATTGGTATGTACCCAGA	36.4	47.6	56.9	56.6	443-447 bp	1.0	41.8
ccsA-ndhD_p5	ACAAATTCAGCAATTGTAGCCT	TCGGTATTGGTATGTACCCAG	36.4	47.6	56.9	56.6	444-448 bp	1.0	41.8