Hotspot

rpl14-rpl16

LSC · 80007-80137 · 131 bp

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Pi 0.0305

MarkerSeek score 70.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 131
variable_sites: 4
pi: 0.0305
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ATCGGCGATTACTAGCTCCT	GTTAAACCGGGGCGAATACT	50.0	50.0	58.1	58.0	345 bp	1.0	80.0
rpl14-rpl16_p2	CCGCTGTTATCCGCTACATT	GTTAAACCGGGGCGAATACT	50.0	50.0	58.1	58.0	295 bp	1.0	79.8
rpl14-rpl16_p3	ATCGGCGATTACTAGCTCCT	AGCAGCATCCAAAATGCCTA	50.0	45.0	58.1	57.9	274 bp	1.0	79.3
rpl14-rpl16_p4	ATCGGCGATTACTAGCTCCT	TAGCAGCATCCAAAATGCCT	50.0	45.0	58.1	57.9	275 bp	1.0	79.3
rpl14-rpl16_p5	CCGCTGTTATCCGCTACATT	AGCAGCATCCAAAATGCCTA	50.0	45.0	58.1	57.9	224 bp	1.0	79.2