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psbT-psbN

LSC · 73313-73375 · 63 bp

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Pi 0.0635

MarkerSeek score 69.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 4
pi: 0.0635
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	GTACGTCGTATGCCCACTTT	GGTGACAGATCCAGCTTCTG	50.0	55.0	58.0	58.0	1450 bp	0.5	59.8
psbT-psbN_p2	TTCTTCGGACACATTTGGCA	GGTGACAGATCCAGCTTCTG	45.0	55.0	58.0	58.0	1129 bp	0.5	59.6
psbT-psbN_p3	GTACGTCGTATGCCCACTTT	AGGTGACAGATCCAGCTTCT	50.0	50.0	58.0	58.1	1451 bp	0.5	59.6
psbT-psbN_p4	TTCTTCGGACACATTTGGCA	AGGTGACAGATCCAGCTTCT	45.0	50.0	58.0	58.1	1130 bp	0.5	59.4
psbT-psbN_p5	ACACATTTGGCATGGTGCTA	GGTGACAGATCCAGCTTCTG	45.0	55.0	58.1	58.0	1121 bp	0.5	59.2