Hotspot

petL-petG

LSC · 65206-65375 · 170 bp

Back to Satureja

Pi 0.0294

MarkerSeek score 69.40

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 5
pi: 0.0294
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGGTCTGAGCAAGATACGAC	TCAAAGGTCCAACTGATCGC	50.0	50.0	57.1	58.2	312-314 bp	1.0	72.2
petL-petG_p2	TCATTTAGCCCCTTCATGCT	TCAAAGGTCCAACTGATCGC	45.0	50.0	56.9	58.2	395-397 bp	1.0	70.4
petL-petG_p3	TTGGTCTGAGCAAGATACGAC	TCAAAGGTCCAACTGATCGC	47.6	50.0	57.8	58.2	313-315 bp	1.0	69.9
petL-petG_p4	GGTCTGAGCAAGATACGACT	TCAAAGGTCCAACTGATCGC	50.0	50.0	56.8	58.2	311-313 bp	1.0	69.8
petL-petG_p5	TCATTTAGCCCCTTCATGCTT	TCAAAGGTCCAACTGATCGC	42.9	50.0	57.6	58.2	395-397 bp	1.0	68.4