Hotspot

trnS-UGA-psbZ

LSC · 34945-35288 · 344 bp

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Pi 0.0118

MarkerSeek score 61.90

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 4
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	458-464 bp	1.0	79.9
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	460-466 bp	1.0	79.5
trnS-UGA-psbZ_p3	GTTTTCAAGACCGGGGCTAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.9	57.8	478-484 bp	1.0	79.0
trnS-UGA-psbZ_p4	CAACCACTCAGCCATCTCTC	GCAAATACGACAGGTACGCT	55.0	50.0	58.0	58.4	442-448 bp	1.0	78.7
trnS-UGA-psbZ_p5	GTTTTCAAGACCGGGGCTAT	GACCAACCATCAGGAGAAGC	50.0	55.0	57.9	58.3	480-486 bp	1.0	78.6