Hotspot

rps16-trnQ-UUG

LSC · 5982-6956 · 975 bp

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Pi 0.0105

MarkerSeek score 57.70

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 975
variable_sites: 10
pi: 0.0105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1032-1049 bp	1.0	80.8
rps16-trnQ-UUG_p2	GCCAAGAGCACCTCATTTCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1103-1120 bp	1.0	80.4
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GTCTCGCTATTCGGAGGTTC	45.0	55.0	58.1	57.9	1045-1062 bp	1.0	80.4
rps16-trnQ-UUG_p4	GCCAAGAGCACCTCATTTCT	GTCTCGCTATTCGGAGGTTC	50.0	55.0	58.2	57.9	1116-1133 bp	1.0	80.0
rps16-trnQ-UUG_p5	AAAAATCCACAACGGGTCGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.3	58.1	1059-1076 bp	1.0	79.3