Hotspot

ycf2

LSC · 89877-96755 · 6879 bp

Back to Sassafras

Pi 0.00297

MarkerSeek score 46.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6879
variable_sites: 28
pi: 0.00297
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGAGTCACAGGTATCTGGCA	TCCGTTGTGGAAGAATCGAC	50.0	50.0	58.1	57.9	638 bp	1.0	80.3
ycf2_p2	TGAGTCACAGGTATCTGGCA	TGATTTGACCCCATAAGCGG	50.0	50.0	58.1	57.9	1081 bp	1.0	80.0
ycf2_p3	TGAGTCACAGGTATCTGGCA	CCACCACCCAATAGCATGTT	50.0	50.0	58.1	58.1	1059 bp	1.0	79.2
ycf2_p4	AGACATTTCTGGAACGCCTG	TGATTTGACCCCATAAGCGG	50.0	50.0	58.2	57.9	952 bp	0.333333	51.4
ycf2_p5	CAGACATTTCTGGAACGCCT	TGATTTGACCCCATAAGCGG	50.0	50.0	58.2	57.9	953 bp	0.333333	51.4