Hotspot

ycf1

SSC · 128277-133898 · 5622 bp

Back to Sassafras

Pi 0.00623
MarkerSeek score 35.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5622
variable_sites
52
pi
0.00623
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
35.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGTCGAAATAAAGTGCATTTT TTCATACGCAAATGGCACTA 28.6 40.0 52.3 55.5 195 bp 1.0 42.5
ycf1_p2 AGTCGAAATAAAGTGCATTTT TCATACGCAAATGGCACTAT 28.6 40.0 52.3 55.2 194 bp 1.0 42.5
ycf1_p3 AGTCGAAATAAAGTGCATTTT TCATACGCAAATGGCACTATT 28.6 38.1 52.3 56.0 194 bp 1.0 42.5
ycf1_p4 AGTCGAAATAAAGTGCATTTT ATTCATACGCAAATGGCACT 28.6 40.0 52.3 56.1 196 bp 1.0 42.4
ycf1_p5 TAGTCGAAATAAAGTGCATTTT ATTCATACGCAAATGGCACT 27.3 40.0 52.4 56.1 197 bp 1.0 42.4