Hotspot

rpoA

LSC · 81458-82477 · 1020 bp

Back to Sassafras

Pi 0.00261

MarkerSeek score 60.40

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 4
pi: 0.00261
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	AGGATTCAAAAGCCATTTTCTTCT	CATAATGGATGTAGGCCTCCT	33.3	47.6	57.3	56.6	1190-1195 bp	1.0	40.3
rpoA_p2	AGGATTCAAAAGCCATTTTCTTCT	TGGATGTAGGCCTCCTAAAA	33.3	45.0	57.3	55.5	1185-1190 bp	1.0	40.3
rpoA_p3	AGGATTCAAAAGCCATTTTCTTCT	TGGATGTAGGCCTCCTAAAAA	33.3	42.9	57.3	56.3	1185-1190 bp	1.0	40.3
rpoA_p4	AGGATTCAAAAGCCATTTTCTTC	CATAATGGATGTAGGCCTCCT	34.8	47.6	56.0	56.6	1190-1195 bp	1.0	40.3
rpoA_p5	GGATTCAAAAGCCATTTTCTTCT	CATAATGGATGTAGGCCTCCT	34.8	47.6	56.0	56.6	1189-1194 bp	1.0	40.3