Hotspot

rps15-ycf1

SSC · 127957-128276 · 320 bp

Back to Sassafras

Pi 0.0167

MarkerSeek score 62.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 320
variable_sites: 8
pi: 0.0167
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TGAAATTCAACAGACCCTTTGT	AATGGCAGCCGTTTCAGTAT	36.4	45.0	56.4	57.9	448-450 bp	1.0	51.6
rps15-ycf1_p2	TGAAATTCAACAGACCCTTTGT	ATAATGGCAGCCGTTTCAGT	36.4	45.0	56.4	57.9	450-452 bp	1.0	51.6
rps15-ycf1_p3	ACTTGAAATTCAACAGACCCT	AATGGCAGCCGTTTCAGTAT	38.1	45.0	55.3	57.9	451-453 bp	1.0	51.1
rps15-ycf1_p4	ACTTGAAATTCAACAGACCCT	ATAATGGCAGCCGTTTCAGT	38.1	45.0	55.3	57.9	453-455 bp	1.0	51.1
rps15-ycf1_p5	TGAAATTCAACAGACCCTTTGT	CCAATAATGGCAGCCGTTTC	36.4	50.0	56.4	57.8	453-455 bp	1.0	50.9