Hotspot

rpl32-trnL-UAG

SSC · 117296-118669 · 1374 bp

Back to Sassafras

Pi 0.00464

MarkerSeek score 62.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1374
variable_sites: 9
pi: 0.00464
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGCGGCAAAAGCTTTATCTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.7	57.9	1507-1517 bp	1.0	77.8
rpl32-trnL-UAG_p2	CGCGGCAAAAGCTTTATCTT	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.7	57.8	1500-1510 bp	1.0	77.3
rpl32-trnL-UAG_p3	CGCGGCAAAAGCTTTATCTT	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.7	57.8	1506-1516 bp	1.0	77.2
rpl32-trnL-UAG_p4	CGCGGCAAAAGCTTTATCTT	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.7	57.8	1505-1515 bp	1.0	77.2
rpl32-trnL-UAG_p5	CGCGGCAAAAGCTTTATCTT	GAGCAGCGTGTCTACCAATT	45.0	50.0	57.7	58.3	1501-1511 bp	1.0	76.6