Hotspot

petA-psbJ

LSC · 66363-67498 · 1136 bp

Back to Sassafras

Pi 0.0413

MarkerSeek score 86.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1136
variable_sites: 69
pi: 0.0413
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	GGCTCATATTCCGGATTGGG	45.0	55.0	58.2	58.2	1257-1281 bp	1.0	83.1
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	CGGCTCATATTCCGGATTGG	45.0	55.0	58.2	58.6	1258-1282 bp	1.0	80.0
petA-psbJ_p3	ACGTGTCCAAGGTTTGTTGT	GCTCATATTCCGGATTGGGA	45.0	50.0	58.2	57.1	1256-1280 bp	1.0	77.6
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	1250-1274 bp	1.0	77.4
petA-psbJ_p5	CGTGTCCAAGGTTTGTTGTT	GGCTCATATTCCGGATTGGG	45.0	55.0	57.1	58.2	1256-1280 bp	1.0	77.0