Hotspot

trnK-UUU-rps16

LSC · 4372-5188 · 817 bp

Back to Sassafras

Pi 0.00408

MarkerSeek score 60.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 817
variable_sites: 4
pi: 0.00408
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	947-949 bp	1.0	79.0
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	946-948 bp	1.0	79.0
trnK-UUU-rps16_p3	AAAAGACCATAGCCGCACTT	AAAAAGGCGCTCAACCTACA	45.0	45.0	57.8	58.0	968-970 bp	1.0	78.8
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	956-958 bp	1.0	78.1
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	50.0	50.0	58.2	58.2	950-952 bp	1.0	77.4