Hotspot

ndhA

SSC · 114126-116240 · 2115 bp

Back to Sasamorpha

Pi 0.00284

MarkerSeek score 37.20

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2115
variable_sites: 9
pi: 0.00284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGTTTGAGAGAACTTTTGTGTTGA	ATGGGGGAAGTTGATCGTTG	33.3	50.0	57.4	57.9	2187-2192 bp	1.0	42.9
ndhA_p2	TGTTTGAGAGAACTTTTGTGTTGA	GGGGGAAGTTGATCGTTGAA	33.3	50.0	57.4	57.8	2185-2190 bp	1.0	42.4
ndhA_p3	TCTTGTTTGAGAGAACTTTTGTGT	ATGGGGGAAGTTGATCGTTG	33.3	50.0	57.2	57.9	2190-2195 bp	1.0	40.9
ndhA_p4	TCTTGTTTGAGAGAACTTTTGTGT	GGGGGAAGTTGATCGTTGAA	33.3	50.0	57.2	57.8	2188-2193 bp	1.0	40.5
ndhA_p5	TGTTTGAGAGAACTTTTGTGTTG	ATGGGGGAAGTTGATCGTTG	34.8	50.0	56.1	57.9	2187-2192 bp	1.0	40.5