Hotspot

rpl14-rpl16

LSC · 79572-79682 · 111 bp

Back to Sasamorpha

Pi 0.0183
MarkerSeek score 54.90
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
111
variable_sites
3
pi
0.0183
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 AAGGGTCTGAGGTTGAATCA TAGCTGCCAGTAAAATGCCC 45.0 50.0 56.0 58.2 182-185 bp 1.0 65.1
rpl14-rpl16_p2 AAAGGGTCTGAGGTTGAATCA TAGCTGCCAGTAAAATGCCC 42.9 50.0 56.8 58.2 183-186 bp 1.0 63.4
rpl14-rpl16_p3 AGGGTCTGAGGTTGAATCAT TAGCTGCCAGTAAAATGCCC 45.0 50.0 55.8 58.2 181-184 bp 1.0 63.2
rpl14-rpl16_p4 AAGGGTCTGAGGTTGAATCAT TAGCTGCCAGTAAAATGCCC 42.9 50.0 56.6 58.2 182-185 bp 1.0 61.6
rpl14-rpl16_p5 AAAAGGGTCTGAGGTTGAATCA TAGCTGCCAGTAAAATGCCC 40.9 50.0 57.5 58.2 184-187 bp 1.0 61.1