Hotspot

psaI-ycf4

LSC · 59131-59500 · 370 bp

Back to Sasamorpha

Pi 0.00551

MarkerSeek score 44.10

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 370
variable_sites: 3
pi: 0.00551
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	GCCCCAACCGATAAAAATCC	45.0	50.0	58.0	57.1	543-550 bp	1.0	74.2
psaI-ycf4_p2	GGCAATTGCAATGGCTTCTT	GCCCCAACCGATAAAAATCC	45.0	50.0	57.9	57.1	537-544 bp	1.0	73.6
psaI-ycf4_p3	ATTTCCGGCAATTGCAATGG	TCCATATGTGTTCTGATCGCC	45.0	47.6	58.0	57.9	452-459 bp	1.0	72.6
psaI-ycf4_p4	ATTTCCGGCAATTGCAATGG	CCATATGTGTTCTGATCGCCA	45.0	47.6	58.0	58.2	451-458 bp	1.0	72.2
psaI-ycf4_p5	GGCAATTGCAATGGCTTCTT	TCCATATGTGTTCTGATCGCC	45.0	47.6	57.9	57.9	446-453 bp	1.0	72.1