Hotspot

rbcL-accD

LSC · 57728-58264 · 537 bp

Back to Sasamorpha

Pi 0.00869

MarkerSeek score 36.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 537
variable_sites: 7
pi: 0.00869
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GGCTTCAGGGGGTATTCATG	GATATCCCCCAACCCCAGTA	55.0	55.0	58.0	57.9	1105 bp	1.0	79.8
rbcL-accD_p2	GGCTTCAGGGGGTATTCATG	TAAGGATATCCCCCAACCCC	55.0	55.0	58.0	57.9	1109 bp	1.0	79.7
rbcL-accD_p3	GGCTTCAGGGGGTATTCATG	CCCAGTATACCGAAACTCGC	55.0	55.0	58.0	58.2	1092 bp	1.0	79.6
rbcL-accD_p4	CTTAACTGGGGGATTCACCG	GATATCCCCCAACCCCAGTA	55.0	55.0	58.0	57.9	1429 bp	1.0	79.4
rbcL-accD_p5	CGCCGGTACAGTAGTAGGTA	GATATCCCCCAACCCCAGTA	55.0	55.0	58.0	57.9	1255 bp	1.0	79.4