Hotspot

matK-trnK

LSC · 3232-3921 · 690 bp

Back to Sasamorpha

Pi 0.00386

MarkerSeek score 59.40

Primer available yes

Label matK-trnK

Feature Metadata

label_name: matK-trnK
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 4
pi: 0.00386
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK_p1	ACTCCTGAAAGAGAAGTGGGT	CGTGCGGTGTAATTCCATTG	47.6	50.0	58.3	57.8	859-865 bp	1.0	68.6
matK-trnK_p2	ACTCCTGAAAGAGAAGTGGG	CGTGCGGTGTAATTCCATTG	50.0	50.0	56.5	57.8	859-865 bp	1.0	66.7
matK-trnK_p3	CTCCTGAAAGAGAAGTGGGT	CGTGCGGTGTAATTCCATTG	50.0	50.0	56.5	57.8	858-864 bp	1.0	66.7
matK-trnK_p4	ACTCCTGAAAGAGAAGTGGGT	TGGGTTGCTAACTCAATGGT	47.6	45.0	58.3	57.0	800-806 bp	1.0	62.2
matK-trnK_p5	ACTCCTGAAAGAGAAGTGGGTA	CGTGCGGTGTAATTCCATTG	45.5	50.0	58.1	57.8	859-865 bp	1.0	61.9