Hotspot

rpl16

LSC · 79690-81191 · 1502 bp

Back to Sasaella

Pi 0.004

MarkerSeek score 48.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1502
variable_sites: 6
pi: 0.004
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTTCGAGCTCCGCTGTTATC	GGACTCAGGCTCAAGTTCTG	50.0	55.0	58.1	57.9	2309-2311 bp	1.0	79.2
rpl16_p2	TAACCGCTGCATTGTCATCA	GGACTCAGGCTCAAGTTCTG	45.0	55.0	57.9	57.9	2515-2517 bp	1.0	78.9
rpl16_p3	TGATAACCGCTGCATTGTCA	GGACTCAGGCTCAAGTTCTG	45.0	55.0	57.9	57.9	2518-2520 bp	1.0	78.9
rpl16_p4	ATCAATTTTCGAGCTCCGCT	GGACTCAGGCTCAAGTTCTG	45.0	55.0	58.0	57.9	2317 bp	0.5	59.0
rpl16_p5	CATCAATTTTCGAGCTCCGC	GGACTCAGGCTCAAGTTCTG	50.0	55.0	57.9	57.9	2318 bp	0.5	58.5