Hotspot

ndhG

SSC · 112747-113277 · 531 bp

Back to Sasaella

Pi 0.00377

MarkerSeek score 54.50

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 2
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGTTGGCAGTAGATGGGTTT	TCCCTTTTTCCTTCCTTGAATCT	45.0	39.1	57.0	57.7	691-710 bp	1.0	45.8
ndhG_p2	TTGTTGGCAGTAGATGGGTT	TCCCTTTTTCCTTCCTTGAATCT	45.0	39.1	57.0	57.7	692-711 bp	1.0	45.8
ndhG_p3	TTTGTTGGCAGTAGATGGGT	TCCCTTTTTCCTTCCTTGAATCT	45.0	39.1	57.0	57.7	693-712 bp	1.0	45.8
ndhG_p4	TGTTGGCAGTAGATGGGTTT	TCCTAATCCCTTTTTCCTTCCT	45.0	40.9	57.0	56.4	697-716 bp	1.0	43.7
ndhG_p5	TTGTTGGCAGTAGATGGGTT	TCCTAATCCCTTTTTCCTTCCT	45.0	40.9	57.0	56.4	698-717 bp	1.0	43.7