Hotspot

rpl33-rps18

LSC · 67292-67553 · 262 bp

Back to Sasaella

Pi 0.0154

MarkerSeek score 61.50

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 262
variable_sites: 4
pi: 0.0154
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGCCACAATACACCC	TGACTGGGCTTGAAATTGCT	50.0	45.0	58.0	58.0	705-727 bp	1.0	80.5
rpl33-rps18_p2	CAAGCTAAGTCAGGCCTCAG	TGACTGGGCTTGAAATTGCT	55.0	45.0	58.0	58.0	1078-1100 bp	1.0	80.3
rpl33-rps18_p3	CATAGAAAGAGGCTGGGCTG	TGACTGGGCTTGAAATTGCT	55.0	45.0	58.0	58.0	1049-1071 bp	1.0	80.2
rpl33-rps18_p4	AGAATCGCCACAATACACCC	CGAAAAGTTTGCTTGGATTTACG	50.0	39.1	58.0	57.5	464-486 bp	1.0	52.9
rpl33-rps18_p5	CCACAATACACCCGGACAAT	CGAAAAGTTTGCTTGGATTTACG	50.0	39.1	57.9	57.5	457-479 bp	1.0	52.2