Hotspot

ndhC-trnV-UAC

LSC · 51835-52578 · 744 bp

Back to Sasaella

Pi 0.00806

MarkerSeek score 63.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 6
pi: 0.00806
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTCACTAATCGGGGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	877-909 bp	1.0	76.2
ndhC-trnV-UAC_p2	CTGGTCCTTCACTAATCGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.4	57.9	881-913 bp	1.0	75.1
ndhC-trnV-UAC_p3	TCCTTCACTAATCGGGGCTA	CGGTTCGAATCCGTATAGCC	50.0	55.0	57.5	58.3	878-910 bp	1.0	74.4
ndhC-trnV-UAC_p4	TGGTCCTTCACTAATCGGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.8	57.9	880-912 bp	1.0	73.5
ndhC-trnV-UAC_p5	CTGGTCCTTCACTAATCGGG	CGGTTCGAATCCGTATAGCC	55.0	55.0	57.4	58.3	882-914 bp	1.0	73.3