Hotspot

rps14-psaB

LSC · 38507-38661 · 155 bp

Back to Sasaella

Pi 0.0195

MarkerSeek score 64.30

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 155
variable_sites: 3
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	CTCAAGGGGGAAACTTTGCT	TGGATTAGCCCACTTTTCCG	50.0	50.0	58.0	57.9	352-353 bp	1.0	79.9
rps14-psaB_p2	CTCAAGGGGGAAACTTTGCT	GGATTAGCCCACTTTTCCGT	50.0	50.0	58.0	57.9	351-352 bp	1.0	79.9
rps14-psaB_p3	CTCAAGGGGGAAACTTTGCT	GCAGCTTTCTTGATTGCCTC	50.0	50.0	58.0	57.7	312-313 bp	1.0	78.8
rps14-psaB_p4	CTCAAGGGGGAAACTTTGCT	GCTCTTTCCATTGTGCAAGC	50.0	50.0	58.0	58.3	381-382 bp	1.0	78.5
rps14-psaB_p5	CTCAAGGGGGAAACTTTGCT	TGGCTCTTTCCATTGTGCAA	50.0	45.0	58.0	58.3	383-384 bp	1.0	78.4