Hotspot

rps4

LSC · 47449-48054 · 606 bp

Pi 0.00413

MarkerSeek score 37.30

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: LSC
length_bp: 606
variable_sites: 5
pi: 0.00413
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	AGGTCGATTTAGTTCGGGGA	TCAATAAAATAAACAAGGAGTCTT	50.0	25.0	58.1	52.6	700 bp	1.0	40.7
rps4_p2	AGGTCGATTTAGTTCGGGGA	TTCAATAAAATAAACAAGGAGTCT	50.0	25.0	58.1	52.6	701 bp	1.0	40.7
rps4_p3	AGGTCGATTTAGTTCGGGGA	TCAATAAAATAAACAAGGAGTCTTC	50.0	28.0	58.1	53.8	700 bp	0.75	30.1
rps4_p4	TTAGTTCGGGGAAAGGGGAA	TCAATAAAATAAACAAGGAGTCTTC	50.0	28.0	58.3	53.8	692 bp	0.75	30.1
rps4_p5	TTTAGTTCGGGGAAAGGGGA	TCAATAAAATAAACAAGGAGTCTTC	50.0	28.0	58.3	53.8	693 bp	0.75	30.1