Hotspot

petB

LSC · 73905-75370 · 1466 bp

Back to Sasa

Pi 0.000682
MarkerSeek score 50.20
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1466
variable_sites
2
pi
0.000682
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 GGTAGTTCGACCGTGGAATT ACCACAACCTACCCGATATGA 50.0 47.6 57.6 58.2 1572-1574 bp 1.0 67.3
petB_p2 GGTAGTTCGACCGTGGAATT ACCACAACCTACCCGATATG 50.0 50.0 57.6 56.7 1572-1574 bp 1.0 67.0
petB_p3 GGTAGTTCGACCGTGGAATT TGAAATACCACAACCTACCCG 50.0 47.6 57.6 57.7 1578-1580 bp 1.0 66.6
petB_p4 ATTCTGGTAGTTCGACCGTG ACCACAACCTACCCGATATGA 50.0 47.6 57.4 58.2 1577-1579 bp 1.0 65.1
petB_p5 CATTCTGGTAGTTCGACCGT ACCACAACCTACCCGATATGA 50.0 47.6 57.4 58.2 1578-1580 bp 1.0 65.1