Hotspot

atpF

LSC · 35347-36746 · 1400 bp

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Pi 0.00155
MarkerSeek score 51.30
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
1400
variable_sites
4
pi
0.00155
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 AAAAAGGGCGAGCGAAGTAA TTCGGAAGCAAGGGGAAAAA 45.0 45.0 58.1 57.9 1535-1544 bp 1.0 79.3
atpF_p2 AAAAAGGGCGAGCGAAGTAA TTTCGGAAGCAAGGGGAAAA 45.0 45.0 58.1 57.9 1536-1545 bp 1.0 79.3
atpF_p3 AAAAAGGGCGAGCGAAGTAA TTTTCGGAAGCAAGGGGAAA 45.0 45.0 58.1 57.9 1537-1546 bp 1.0 79.3
atpF_p4 AAAAAGGGCGAGCGAAGTAA TTTTTCGGAAGCAAGGGGAA 45.0 45.0 58.1 57.9 1538-1547 bp 1.0 79.3
atpF_p5 AAAAAGGGCGAGCGAAGTAA TCGAAGGGTTGCCATTAGTG 45.0 50.0 58.1 57.9 1571-1580 bp 1.0 79.0