Hotspot

ndhC-trnV-UAC

LSC · 52549-53311 · 763 bp

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Pi 0.00218
MarkerSeek score 53.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
763
variable_sites
3
pi
0.00218
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCCTTCACTAATCGGGGCTA GGTTCGAATCCGTATAGCCC 50.0 55.0 57.5 57.9 877-896 bp 1.0 77.6
ndhC-trnV-UAC_p2 CTGGTCCTTCACTAATCGGG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.4 57.9 881-900 bp 1.0 76.5
ndhC-trnV-UAC_p3 TCCTTCACTAATCGGGGCTA CGGTTCGAATCCGTATAGCC 50.0 55.0 57.5 58.3 878-897 bp 1.0 75.8
ndhC-trnV-UAC_p4 TGGTCCTTCACTAATCGGGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.8 57.9 880-899 bp 1.0 75.0
ndhC-trnV-UAC_p5 CTGGTCCTTCACTAATCGGG CGGTTCGAATCCGTATAGCC 55.0 55.0 57.4 58.3 882-901 bp 1.0 74.7