Hotspot

trnT-UGU-trnL-UAA

LSC · 48430-49248 · 819 bp

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Pi 0.00183
MarkerSeek score 60.40
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
819
variable_sites
3
pi
0.00183
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 918-923 bp 1.0 80.3
trnT-UGU-trnL-UAA_p2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.1 58.0 910-915 bp 1.0 79.9
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 55.0 50.0 57.9 58.0 888-893 bp 1.0 79.4
trnT-UGU-trnL-UAA_p4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 50.0 55.0 58.0 58.2 908-913 bp 1.0 78.3
trnT-UGU-trnL-UAA_p5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 50.0 50.0 58.0 57.7 917-922 bp 1.0 78.1