Hotspot

rps14-psaB

LSC · 39248-39402 · 155 bp

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Pi 0.0129
MarkerSeek score 52.80
Primer available yes
Label rps14-psaB

Feature Metadata

label_name
rps14-psaB
feature_type
igs
region
LSC
length_bp
155
variable_sites
4
pi
0.0129
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps14-psaB_p1 CTCAAGGGGGAAACTTTGCT TGGATTAGCCCACTTTTCCG 50.0 50.0 58.0 57.9 352-353 bp 1.0 80.1
rps14-psaB_p2 CTCAAGGGGGAAACTTTGCT GGATTAGCCCACTTTTCCGT 50.0 50.0 58.0 57.9 351-352 bp 1.0 80.1
rps14-psaB_p3 CTCAAGGGGGAAACTTTGCT GCAGCTTTCTTGATTGCCTC 50.0 50.0 58.0 57.7 312-313 bp 1.0 79.1
rps14-psaB_p4 CTCAAGGGGGAAACTTTGCT GCTCTTTCCATTGTGCAAGC 50.0 50.0 58.0 58.3 381-382 bp 1.0 78.7
rps14-psaB_p5 CTCAAGGGGGAAACTTTGCT TGGCTCTTTCCATTGTGCAA 50.0 45.0 58.0 58.3 383-384 bp 1.0 78.7