Hotspot

rpoC2-rps2

LSC · 31775-32100 · 326 bp

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Pi 0.0327
MarkerSeek score 77.30
Primer available yes
Label rpoC2-rps2

Feature Metadata

label_name
rpoC2-rps2
feature_type
igs
region
LSC
length_bp
326
variable_sites
17
pi
0.0327
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-rps2_p1 ATGAGACATCAGAACCCCCA ACCAAAATGAACTCCCGCTT 50.0 45.0 58.0 58.0 408-433 bp 1.0 87.0
rpoC2-rps2_p2 TGAGACATCAGAACCCCCAT ACCAAAATGAACTCCCGCTT 50.0 45.0 58.0 58.0 407-432 bp 1.0 87.0
rpoC2-rps2_p3 TGAGACATCAGAACCCCCAT TTTACGCTTTGCCGAGATGT 50.0 45.0 58.0 58.2 464-489 bp 1.0 85.1
rpoC2-rps2_p4 ATGAGACATCAGAACCCCCA TTTACGCTTTGCCGAGATGT 50.0 45.0 58.0 58.2 465-490 bp 1.0 85.0
rpoC2-rps2_p5 TGAGACATCAGAACCCCCAT CTTTGCCGAGATGTAAGGGG 50.0 55.0 58.0 58.3 458-483 bp 1.0 84.0