Hotspot

trnC-GCA-rpoB

LSC · 20534-21710 · 1177 bp

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Pi 0.00255
MarkerSeek score 52.10
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1177
variable_sites
6
pi
0.00255
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1261-1263 bp 1.0 77.2
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1260-1262 bp 1.0 77.2
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG AAGTTCTTCTGCCAAGCCTT 50.0 45.0 58.1 57.6 1325-1327 bp 1.0 76.8
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG AGTTCTTCTGCCAAGCCTTG 50.0 50.0 58.1 58.4 1324-1326 bp 1.0 76.7
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG CAAGTTCTTCTGCCAAGCCT 50.0 50.0 58.1 58.4 1326-1328 bp 1.0 76.7