Hotspot

rps3

LSC · 78864-79538 · 675 bp

Back to Sartidia

Pi 0.00494

MarkerSeek score 50.00

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 675
variable_sites: 5
pi: 0.00494
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	GGACAGAATGGGAAAACAAGC	TCTAAGATTTCGATTCCCAGT	47.6	38.1	57.7	54.1	743 bp	1.0	40.5
rps3_p2	GGACAGAATGGGAAAACAAGC	TCAATCTAAGATTTCGATTCCCAGT	47.6	36.0	57.7	58.1	747 bp	1.0	40.5
rps3_p3	GGACAGAATGGGAAAACAAGC	CAATCTAAGATTTCGATTCCCAGT	47.6	37.5	57.7	56.8	746 bp	1.0	40.5
rps3_p4	ACTGGACAGAATGGGAAAACA	TCTAAGATTTCGATTCCCAGT	42.9	38.1	57.4	54.1	746 bp	1.0	40.5
rps3_p5	TCACTGGACAGAATGGGAAA	TCTAAGATTTCGATTCCCAGT	45.0	38.1	56.4	54.1	748 bp	1.0	40.5