Hotspot

ndhF-rpl32

SSC · 105038-105759 · 722 bp

Back to Sartidia

Pi 0.0106
MarkerSeek score 61.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
722
variable_sites
11
pi
0.0106
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TCCAAAGATTTTTACGAATACGCT 55.0 33.3 58.1 57.3 813-834 bp 1.0 44.8
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TGACATCGAAGTACGTTTTT 55.0 35.0 58.1 53.2 785-806 bp 1.0 44.7
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTGACATCGAAGTACGTTTT 55.0 35.0 58.1 53.2 786-807 bp 1.0 44.7
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTGACATCGAAGTACGTTT 55.0 35.0 58.1 53.2 787-808 bp 1.0 44.7
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TTTTGACATCGAAGTACGTT 55.0 35.0 58.1 53.2 788-809 bp 1.0 44.6