Hotspot

trnL-UAA-trnF-GAA

LSC · 47424-47724 · 301 bp

Back to Sartidia

Pi 0.0155

MarkerSeek score 47.50

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 301
variable_sites: 7
pi: 0.0155
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	GACGCTACGGACTTGATTGT	TTCTGGGTTTGGAGAAGTGC	50.0	50.0	58.0	58.0	1627-1643 bp	1.0	80.8
trnL-UAA-trnF-GAA_p2	GACGCTACGGACTTGATTGT	TTTGAACTGGTGACACGAGG	50.0	50.0	58.0	58.1	958-972 bp	1.0	80.5
trnL-UAA-trnF-GAA_p3	GACGCTACGGACTTGATTGT	CGCGCGCATTCATTACTTAG	50.0	50.0	58.0	58.1	1199-1211 bp	1.0	80.5
trnL-UAA-trnF-GAA_p4	GGTCTAGACGAGCTGCTAGA	TTCTGGGTTTGGAGAAGTGC	55.0	50.0	58.0	58.0	1758-1774 bp	1.0	80.4
trnL-UAA-trnF-GAA_p5	AATAAGCGGGGTCTAGACGA	TTCTGGGTTTGGAGAAGTGC	50.0	50.0	57.9	58.0	1767-1783 bp	1.0	80.2