Hotspot

psbM-petN

LSC · 16637-17146 · 510 bp

Back to Sartidia

Pi 0.00267

MarkerSeek score 60.60

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 510
variable_sites: 2
pi: 0.00267
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	ACAGCCAGCCAAAACGATTA	TAGTAAGTCTCGCTTGGGCT	45.0	50.0	58.1	58.2	603-614 bp	1.0	79.7
psbM-petN_p2	ACAGCCAGCCAAAACGATTA	GTAGTATGGGGGAGGAGTGG	45.0	60.0	58.1	58.3	547-558 bp	1.0	78.9
psbM-petN_p3	ACAGCCAGCCAAAACGATTA	TAGTATGGGGGAGGAGTGGA	45.0	55.0	58.1	58.4	546-557 bp	1.0	78.1
psbM-petN_p4	AAAAACAGCCAGCCAAAACG	TAGTAAGTCTCGCTTGGGCT	45.0	50.0	58.3	58.2	607-618 bp	1.0	77.7
psbM-petN_p5	ACAGCCAGCCAAAACGATTA	CGCTAGTAGTATGGGGGAGG	45.0	60.0	58.1	58.5	552-563 bp	1.0	77.5