Hotspot

trnD-GUC-psbM

LSC · 15977-16531 · 555 bp

Back to Sartidia

Pi 0.0036

MarkerSeek score 61.10

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 555
variable_sites: 3
pi: 0.0036
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	TTCAATTGGTCAGAGCACCG	TAATCGTTTTGGCTGGCTGT	50.0	45.0	58.5	58.1	723-1176 bp	1.0	76.0
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	CGTTTTGGCTGGCTGTTTTT	50.0	45.0	58.5	58.3	719-1172 bp	1.0	74.0
trnD-GUC-psbM_p3	CCTGCAGGGATTGTAGTTCA	TAATCGTTTTGGCTGGCTGT	50.0	45.0	57.2	58.1	739-1192 bp	1.0	73.6
trnD-GUC-psbM_p4	GTTCAATTGGTCAGAGCACC	TAATCGTTTTGGCTGGCTGT	50.0	45.0	57.0	58.1	724-1177 bp	1.0	71.9
trnD-GUC-psbM_p5	CCTGCAGGGATTGTAGTTCA	CGTTTTGGCTGGCTGTTTTT	50.0	45.0	57.2	58.3	735-1188 bp	1.0	71.5