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trnK-UUU-rps16

LSC · 3920-4497 · 578 bp

Back to Sartidia

Pi 0.00346

MarkerSeek score 60.50

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 578
variable_sites: 3
pi: 0.00346
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	GGTGCTCAACCTACAAGAACT	50.0	47.6	58.6	57.9	704-706 bp	1.0	66.8
trnK-UUU-rps16_p2	TAGTCGCACTTAAAAGCCGA	GGTGCTCAACCTACAAGAACT	45.0	47.6	57.3	57.9	705-707 bp	1.0	65.5
trnK-UUU-rps16_p3	TCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAAGAACT	45.0	47.6	57.3	57.9	702-704 bp	1.0	65.5
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	GGTGCTCAACCTACAAGAACT	50.0	47.6	57.2	57.9	701-703 bp	1.0	65.1
trnK-UUU-rps16_p5	GTCGCACTTAAAAGCCGAGT	GGTGCTCAACCTACAAGAACT	50.0	47.6	58.9	57.9	703-705 bp	1.0	64.5