Hotspot

ycf1-rps15

IRb · 118067-118440 · 374 bp

Back to Sarracenia

Pi 0.314

MarkerSeek score 66.90

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: IRb
length_bp: 374
variable_sites: 50
pi: 0.314
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.43
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TCGACTGCGAAATCCAAGAA	ATAAGCTAGCAGGCGTTGAC	45.0	50.0	57.6	58.1	1221 bp	0.5	55.9
ycf1-rps15_p2	TAATCAAGGATGCCGAGAGC	ATAAGCTAGCAGGCGTTGAC	50.0	50.0	57.5	58.1	1002 bp	0.5	55.3
ycf1-rps15_p3	TCGACTGCGAAATCCAAGAA	ACAAATAAGCTAGCAGGCGT	45.0	45.0	57.6	57.6	1225 bp	0.5	53.2
ycf1-rps15_p4	TCGACTGCGAAATCCAAGAA	CGGCTTCTTGAAGGTCACAA	45.0	50.0	57.6	58.4	1707 bp	0.5	53.2
ycf1-rps15_p5	TGGCAGTCGTTTCAGTATGT	ATAAGCTAGCAGGCGTTGAC	45.0	50.0	57.2	58.1	630 bp	0.5	52.9