Hotspot

petN-psbM

LSC · 29881-30908 · 1028 bp

Back to Sarracenia

Pi 0.000973

MarkerSeek score 59.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1028
variable_sites: 1
pi: 0.000973
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.0	57.6	1189 bp	1.0	68.6
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1129 bp	1.0	66.0
petN-psbM_p3	AGTAAATCTCGCTTGGGCTG	TGCTACTGCACTGTTCATTC	50.0	45.0	58.0	56.1	1189 bp	1.0	64.4
petN-psbM_p4	AGTAAATCTCGCTTGGGCTG	GCTACTGCACTGTTCATTCT	50.0	45.0	58.0	55.8	1188 bp	1.0	62.0
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	50.0	45.0	57.6	56.1	1129 bp	1.0	61.8