Hotspot

ycf4

Genome · 63085-63639 · 555 bp

Back to Sarmentypnum

Pi 0.0018

MarkerSeek score 50.40

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: Genome
length_bp: 555
variable_sites: 1
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	ACCTTCGATTTTCGTTCCTCT	ATTATGGCTTGCCAAGACCG	42.9	50.0	57.3	58.6	1258 bp	1.0	61.9
ycf4_p2	ACCTTCGATTTTCGTTCCTCT	AGCTCTTTCCAAAGAACGGT	42.9	45.0	57.3	57.4	1163 bp	1.0	61.6
ycf4_p3	ACCTTCGATTTTCGTTCCTCT	CATTATGGCTTGCCAAGACC	42.9	50.0	57.3	57.1	1259 bp	1.0	59.7
ycf4_p4	ACCTTCGATTTTCGTTCCTCT	AAGCTCTTTCCAAAGAACGGT	42.9	42.9	57.3	58.1	1164 bp	1.0	58.2
ycf4_p5	TCCAGCAATTACAATGGCTTC	ATTATGGCTTGCCAAGACCG	42.9	50.0	56.9	58.6	1222 bp	1.0	58.1